"Probably the fourth or fifth step around third, I felt it tighten up a little bit," Turner said.
As he walked back to the dugout, Turner grabbed the back of his right leg. Josh Satin then replaced him at third base for the top of the seventh inning.
Turner said this is the first time he's experienced a hamstring injury of any kind. The 28-year-old said he'll get an MRI on Wednesday.
"I think I'm going to go get a picture on it tomorrow and find out what exactly is going on in there," Turner said. "Hopefully, it's not too bad, and in a couple of days I'll get back out there."
Turner missed about a month this season with a left intercostal strain. He's hitting .273 with two home runs and 15 RBIs in 81 games this season.
Chris Iseman is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.